Characterization of antibiotic resistance in Listeria spp. isolated from slaughterhouse environments, pork and human infections

Authors

  • Luisa Z Moreno Faculdade de Saúde Pública, Universidade de São Paulo, São Paulo, Brasil
  • Renata Paixão Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brasil
  • Débora D. S. Gobbi Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brasil
  • Daniele C. Raimundo Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brasil
  • Thais P. Ferreira Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brasil
  • Andrea M. Moreno Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brasil
  • Ernesto Hofer Fundação Instituto Oswaldo Cruz, Rio de Janeiro, Brasil
  • Cristhiane M. F. Reis Fundação Instituto Oswaldo Cruz, Rio de Janeiro, Brasil
  • Glavur R. Matté Faculdade de Saúde Pública, Universidade de São Paulo, São Paulo, Brasil
  • Maria H. Matté Faculdade de Saúde Pública, Universidade de São Paulo, São Paulo, Brasil

DOI:

https://doi.org/10.3855/jidc.4188

Keywords:

Listeria, antibiotic resistance, microdilution, PCR

Abstract

Introduction: Listeria species are susceptible to most antibiotics. However, over the last decade, increasing reports of multidrug-resistant Listeria spp. from various sources have prompted public health concerns. The objective of this study was to characterize the antibiotic susceptibility of Listeria spp. and the genetic mechanisms that confer resistance.

Methodology: Forty-six Listeria spp. isolates were studied, and their minimal inhibitory concentrations of antibiotics were determined by microdilution using Sensititrestandard susceptibility MIC plates. The isolates were screened for the presence of gyrA, parC, lde, lsa(A), lnu(A), and mprF by PCR, and the amplified genes were sequenced.

Results: All isolates were susceptible to penicillin, ampicillin, tetracycline, erythromycin, and carbapenems. Resistance to clindamycin, daptomycin, and oxacillin was found among L. monocytogenes and L. innocua, and all species possessed at least intermediate resistance to fluoroquinolones. GyrA, parC, and mprF were detected in all isolates; however, mutations were found only in gyrA sequences. A high daptomycin MIC, as reported previously, was observed, suggesting an intrinsic resistance of Listeria spp. to daptomycin.

Conclusions: These results are consistent with reports of emerging resistance in Listeria spp. and emphasize the need for further genotypic characterization of antibiotic resistance in this genus.

Author Biographies

Luisa Z Moreno, Faculdade de Saúde Pública, Universidade de São Paulo, São Paulo, Brasil

Laboratory of Public Health - Dept of Public Health Practices

Renata Paixão, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brasil

Laboratory of Swine Health

Débora D. S. Gobbi, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brasil

Laboratory of Swine Health

Daniele C. Raimundo, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brasil

Laboratory of Swine Health

Thais P. Ferreira, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brasil

Laboratory of Swine Health

Andrea M. Moreno, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brasil

Laboratory of Swine Health

Ernesto Hofer, Fundação Instituto Oswaldo Cruz, Rio de Janeiro, Brasil

Laboratório de Zoonoses Bacterianas

Cristhiane M. F. Reis, Fundação Instituto Oswaldo Cruz, Rio de Janeiro, Brasil

Laboratório de Zoonoses Bacterianas

Glavur R. Matté, Faculdade de Saúde Pública, Universidade de São Paulo, São Paulo, Brasil

Laboratory of Public Health - Dept of Public Health Practices

Maria H. Matté, Faculdade de Saúde Pública, Universidade de São Paulo, São Paulo, Brasil

Laboratory of Public Health - Dept of Public Health Practices

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Published

2014-04-15

How to Cite

1.
Moreno LZ, Paixão R, Gobbi DDS, Raimundo DC, Ferreira TP, Moreno AM, Hofer E, Reis CMF, Matté GR, Matté MH (2014) Characterization of antibiotic resistance in Listeria spp. isolated from slaughterhouse environments, pork and human infections. J Infect Dev Ctries 8:416–423. doi: 10.3855/jidc.4188

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Section

Original Articles