PCR for M. tuberculosis in tissue samples
Methodology: A PCR-assay specific for IS6110 was evaluated for 104 different tissue samples in comparison to histopathology that was considered gold standard.
Results: PCR showed 74.1% sensitivity and 96.1% specificity. False positive and false negative results were observed in three (2.88%) and seven (6.73%) samples, respectively. Positive agreement between histopathology and PCR was observed as 0.737, indicating substantial good agreement between two tests.
Conclusions: PCR can be used for early diagnosis of tuberculosis in tissue samples that can help to initiate timely anti-tubercular treatment and prevent progression to irreversible changes.