Antibiotic resistance and biofilm formation in children with Enteropathogenic Escherichia coli (EPEC) in Brazilian Amazon

  • Renata Santos Rodrigues Tropical Medicine Research Center, Porto Velho, Rondônia, Brazil
  • Nucia Cristiane da Silva Lima Oswaldo Cruz Foundation, Rondônia, Porto Velho, Rondônia, Brazil
  • Roger Lafontaine Mesquita Taborda Tropical Medicine Research Center, Porto Velho, Rondônia, Brazil
  • Rosimar Pires Esquerdo Oswaldo Cruz Foundation, Rondônia, Porto Velho, Rondônia, Brazil
  • Antonieta Rodrigues Gama Cosme and Damião Children Hospital, Porto Velho, Rondônia, Brazil
  • Paulo Afonso Nogueira Oswaldo Cruz Foundation - Amazonas Leônidas and Maria Deane, Manaus, Amazônia, Brazil
  • Patricia Puccinelli Orlandi Oswaldo Cruz Foundation - Amazonas Leônidas and Maria Deane, Manaus, Amazônia, Brazil
  • Najla Benevides Matos Tropical Medicine Research Center, Porto Velho, Rondônia, Brazil
Keywords: Antibiotic resistance, Biofilm, Enteropathogenic E. coli, Porto Velho-Rondônia

Abstract

Introduction: Enteropathogenic Escherichia coli is an important causative agent of diarrhea in both developed and developing countries.

Methodology: We assessed the antibiotic resistance profile and the ability of 71 Enteropathogenic Escherichia coli (EPEC) isolates from children in the age group 6 years, or younger, to form biofilm. These children were hospitalized in Cosme and Damião Children Hospital in Porto Velho, Western Brazilian Amazon, between 2010 and 2012, with clinical symptoms of acute gastroenteritis.

Results: The highest frequency of atypical EPEC (aEPEC) isolates reached 83.1% (59/71). Most EPEC isolates presented Localized Adherence Like (LAL) pattern in HEp-2 cells (57.7% - 41/71). Biofilm production was observed in 33.8% (24/71) of EPEC isolates, and it means statistically significant association with shf gene (p = 0.0254). The highest antimicrobial resistance rates and a large number of multiresistant isolates 67.6% (48/71), regarded cefuroxime (CXM), ampicillin (AMP), trimethoprim-sulfamethoxazole (SXT) and tetracycline (TET), respectively, mainly in typical EPEC (tEPEC). Furthermore, 96% (68/71) of EPEC isolates in the present study were resistant to at least one antibiotic, whereas only 3 isolates were sensitive to all the tested drugs.

Conclusion: Based on our findings, there was increased aEPEC identification. EPEC isolates showed high resistance rate; most strains showed multiresistance; thus, they work as warning about the continuous need of surveillance towards antimicrobial use. Besides, the ability of forming biofilm was evidenced by the EPEC isolates. This outcome is worrisome, since it is a natural resistance mechanism of bacteria.

Published
2019-08-31
How to Cite
1.
Rodrigues RS, Lima NC da S, Taborda RLM, Esquerdo RP, Gama AR, Nogueira PA, Orlandi PP, Matos NB (2019) Antibiotic resistance and biofilm formation in children with Enteropathogenic Escherichia coli (EPEC) in Brazilian Amazon. J Infect Dev Ctries 13:698-705. doi: 10.3855/jidc.10674
Section
Original Articles