TY - JOUR AU - Muller, Barbara AU - Cunha-Neto, Adelino AU - Castro, Vinicius Silva AU - Carvalho, Ricardo Cesar Tavares AU - Carvalho Teixeira, Larrayane Albuês AU - Rodrigues, Dalia dos Prazeres AU - de Souza Figueiredo, Eduardo Eustáquio PY - 2021/12/31 Y2 - 2024/03/29 TI - Salmonella Schwarzengrund, Akuafo, and O:16 isolated from vacuum-packaged beef produced in the state of Mato Grosso, Brazil JF - The Journal of Infection in Developing Countries JA - J Infect Dev Ctries VL - 15 IS - 12 SE - Original Articles DO - 10.3855/jidc.13726 UR - https://www.jidc.org/index.php/journal/article/view/35044946 SP - 1876-1882 AB - <p>Introduction: <em>Salmonella</em> spp. is a pathogen associated with foodborne infections, mainly in foods of animal origin. In this context, the present study investigated the occurrence of <em>Salmonella</em> serotypes, genotypes and the antimicrobial resistance profiles of strains in fresh beef produced in Mato Grosso, Brazil.</p><p>Methodology: A total of 107 samples from 13 different slaughterhouses in the Mato Grosso were analyzed. Suggestive <em>Salmonella</em> spp. colonies detected during the biochemical screening were submitted to DNA extraction, and <em>hilA</em> gene amplification was used for the PCR reaction. Antimicrobial resistance analyses were performed using 17 antimicrobial agents from eight different classes by the disk diffusion method. Strains exhibiting multiple drug resistances were submitted to PCR genotyping based on repetitive sequences (rep-PCR), using a commercial semiautomatic DiversiLab® system.</p><p>Results: A total of 5.6% (6/107) of the samples tested positive by the conventional method and were confirmed by PCR, namely two <em>S</em>. Akuafo, two non-typable <em>Salmonella</em> enterica strains, one <em>Salmonella</em> O:16 serovar, and one <em>S</em>. Schwarzengrund. The antimicrobial resistance profiles indicated resistance to gentamicin (30%), tetracycline, nitrofurantoin, and trimethoprim + sulfamethoxazole (16%). Genotyping indicated a 70% difference between <em>S</em>. Schwarzengrund and the non-typable <em>Salmonella</em> strains. No genetic similarities were observed between the six <em>Salmonella</em> isolates based on rep-PCR, including two <em>S</em>. Akuafo.</p><p>Conclusions: The results obtained herein corroborate that <em>Salmonella</em> serovar Schwarzengrund is commonly isolated in animal products in the state of Mato Grosso, Brazil, also highlighting the presence of two unusual <em>Salmonella</em> serovars in beef (Akuafo and O:16).</p> ER -