TY - JOUR AU - Ifeanyi, Casmir Ifeanyichukwu Cajetan AU - Bassey, Bassey Enya AU - Ikeneche, Nkiruka Florence AU - Al-Gallas, Nazek PY - 2014/06/11 Y2 - 2024/03/29 TI - Molecular characterization and antibiotic resistance of Salmonella in children with acute gastroenteritis in Abuja, Nigeria JF - The Journal of Infection in Developing Countries JA - J Infect Dev Ctries VL - 8 IS - 06 SE - Original Articles DO - 10.3855/jidc.4185 UR - https://www.jidc.org/index.php/journal/article/view/24916869 SP - 712-719 AB - <p class="SmallText">Introduction: In Nigeria, acute gastroenteritis in children under five years of age is a major cause of mortality and morbidity; identification and characterization of microbial agents of acute gastroenteritis, including <em>Salmonella</em>, remains a powerful tool for effective management, surveillance, and control.</p> <p class="SmallText">Methodology: Diarrheal stool samples were directly plated onto differential and selective media to isolate <em>Salmonella.</em> Extended-spectrum beta-lactamases were screened using the double disk diffusion technique and by PCR targeting the <em>bla</em><sub>TEM </sub>and<em> bla</em><sub>CTX-M </sub>genes. Pulsed-field gel electrophoresis (PFGE) was performed usingthe PulseNet Canada Laboratory protocol for molecular subtyping using the restriction enzymes <em>Xba</em>I and <em>Bln</em>I.</p> <p class="SmallText">Results: The serotypes identified were <em>Salmonella enterica </em>serovar Zanzibar (n = 5), <em>Salmonella </em>Brancaster (n = 3), and one isolate of <em>Salmonella </em>Enteritidis (phage type 1). The following levels of resistance were found among the <em>Salmonella </em>strains: amoxicillin, five strains (55.6%); amoxicillin-clavulanic acid, two strains (22.2%); cephalexin, five strains (55.6%); and cefuroxime, five strains (55.6%). Intermediate resistance was found in five strains (55.6%) only to amoxicillin-clavulanic acid. All isolates were susceptible to nalidixic acid, ciprofloxacin, and ceftriaxone, and no ESBL-producing <em>Salmonella</em> were detected.</p> <p class="SmallText">Conclusions: Our findings demonstrated the involvement of three <em>Salmonella</em> serovars in acute gastroenteritis; resistance to penicillins and cephalosporins was common.</p> ER -