TY - JOUR AU - Sangare, Samba Adama AU - Maiga, Almoustapha Issiaka AU - Guindo, Ibrehima AU - Maiga, Aminata AU - Camara, Namory AU - Dicko, Oumar Agaly AU - Diallo, Souleymane AU - Bougoudogo, Flabou AU - Armand-Lefevre, Laurence AU - Andremont, Antoine AU - Maiga, Ibrahim Izetiegouma PY - 2016/10/31 Y2 - 2024/03/29 TI - Prevalence of ESBL-producing Enterobacteriaceae isolated from blood cultures in Mali JF - The Journal of Infection in Developing Countries JA - J Infect Dev Ctries VL - 10 IS - 10 SE - Original Articles DO - 10.3855/jidc.7536 UR - https://www.jidc.org/index.php/journal/article/view/27801367 SP - 1059-1064 AB - <p class="SmallText">Introduction: The increasing frequency of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae is becoming a serious public health concern. This study sought to determine ESBL frequency in Enterobacteriaceae isolated from patients’ blood cultures in two university teaching hospitals of Bamako, Mali.</p> <p class="SmallText">Methodology: During a three-month period, the presence of Enterobacteriaceae from blood cultures of patients admitted to the university teaching hospitals of Bamako was evaluated. The microbial identifications were initially performed with an API 20E gallery and VITEK2 locally in Mali, and then confirmation in France was performed with a mass spectrometry MALDI-TOF in the bacteriology laboratory of the university teaching hospital of Bichat. Antibiotic susceptibility profiles were determined by the diffusion method as recommended by the European Committee on Antimicrobial Susceptibility Testing (EUCAST).</p> <p class="SmallText">Results: The isolated species were <em>K. pneumoniae</em> (14/40; 35.0%), <em>E. coli</em> (11/40; 27.5%), and <em>E. cloacae</em> (9/40; 22.5%). Of the strains isolated, 21/34 (61.8%) had an ESBL phenotype, including 10/14 (71.4%) <em>K. pneumoniae</em>, 8/11 (72.7%) <em>E. coli</em>, and 3/9 (33.3%) <em>E. cloacae</em>.</p> <p class="SmallText">Resistances associated with ESBL strains of <em>K. pneumoniae</em>, <em>E. coli</em>, and <em>E. cloacae</em> were as follows: gentamicin (10/10, 100%; 6/8, 75%; 2/3, 67%, respectively), amikacin (2/10, 20%; 0/8, 0%; 0/3, 0%, respectively), ofloxacin (8/10, 80%; 7/8, 87%; 3/3, 100%, respectively), and cotrimoxazole (10/10, 100%; 6/8, 75%; 3/3, 100%, respectively).</p> <p class="SmallText">Conclusion: Almost two-thirds (61.8%) of Enterobacteriaceae isolated from our blood cultures were ESBL producers. Only susceptibilities to carbapenems and to amikacin were fully conserved within the strains.</p> ER -