Escherichia coli from community-acquired urinary tract infections resistant to fluoroquinolones and extended-spectrum beta-lactams

Authors

  • Samuel Kariuki Centre for Microbiology Research, Kenya Medical Research Institute, PO Box 43640, Nairobi
  • Gunturu Revathi Department of Pathology, Aga Khan University Hospital, P.O. Box 30270, Nairobi
  • John Corkill Department of Medical Microbiology and Genito-Urinary Medicine, University of Liverpool, Liverpool, L69 3GA
  • John Kiiru Centre for Microbiology Research, Kenya Medical Research Institute, PO Box 43640, Nairobi
  • Joyce Mwituria Centre for Microbiology Research, Kenya Medical Research Institute, PO Box 43640, Nairobi
  • Nazir Mirza The Nairobi Hospital, PO Box 30026, Nairobi
  • C. Anthony Hart Department of Medical Microbiology and Genito-Urinary Medicine, University of Liverpool, Liverpool, L69 3GA

DOI:

https://doi.org/10.3855/jidc.361

Keywords:

Fluoroquinolone-resistant, ESBL-producing MDR uropathogenic E. coli, Kenya

Abstract

Background: Uropathogenic Escherichia coli are increasingly becoming resistant to flouroquinolones and to other commonly available antimicrobials. We sought to investigate the genetic basis for fluoroquinolone and extended spectrum beta-lactam (ESBL) resistance in 17 fluoroquinolone-resistant (MIC of levofloxacin and ciprofloxacin >32 μg/ml) E. coli isolated from patients with urinary tract infections (UTIs). Methods: We applied PCR and Pulsed Field Gel Electrophoresis (PFGE) to characterize resistance genes and to determine clonal relatedness of strains, respectively. Results: Twelve of the 17 E. coli were resistant to multiple drugs, including ampicillin, co-amoxyclav, cefotaxime, ceftriaxone, ceftazidime and gentamicin and nalidixic acid and produced plasmid-mediated CTX-M-15 type ESBLs and CMY-2 AmpC type enzymes. The other 5 E. coli that were non-ESBL-producing were multiply resistant to ampicillin, nitrofurantoin, cefoxitin, nalidixic acid. Resistance to fluoroquinolones resulted from a combination of the presence of qnrA, qnrB, ciprofloxacin acetylating enzyme designated aac(6’)-1b-cr, and mutations in the two amino acid substitutions; 83 Serine (TCG) to Leucine (TTG) and 87 Aspartic acid (GAC) to Asparagine (AAC). Conclusion: Antibiogram patterns and PFGE of E. coli showed that these were community acquired UTI caused by pockets of clonally-related and some discreet strain types. Plasmid-mediated CTX-M-15 beta-lactamases and CMY-2 AmpC enzymes and fluoroquinolone resistant E. coli are becoming increasingly prevalent in hospitals in Kenya, posing a major challenge in the management of UTIs.

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Published

2007-12-01

How to Cite

1.
Kariuki S, Revathi G, Corkill J, Kiiru J, Mwituria J, Mirza N, Hart CA (2007) Escherichia coli from community-acquired urinary tract infections resistant to fluoroquinolones and extended-spectrum beta-lactams. J Infect Dev Ctries 1:257–262. doi: 10.3855/jidc.361

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Original Articles