Isolation, drug resistance and molecular characterization of Salmonella isolates in northern Morocco

  • Sanae Ammari Service de Recherche, Laboratoire de biologie moléculaire, Institut Pasteur du Maroc, Tanger
  • Amine Laglaoui Equipe de Recherche sur la Valorisation Biotechnologique des Microorganisme ERVABIM, Faculté des Sciences et Techniques
  • Latifa En-nanei Service de Recherche, Laboratoire de biologie moléculaire, Institut Pasteur du Maroc, Tanger
  • Sophie Bertrand Bacteriology Division, Scientific Institute of Public Health, J. Wytsman Street, 14 B-1050, Brussels
  • Christa Wildemauwe Pasteur Institute, Phage Typing Service, Engelandstreet 642- B-1180, Brussels
  • Said Barrijal Equipe de Recherche sur la Valorisation Biotechnologique des Microorganisme ERVABIM, Faculté des Sciences et Techniques
  • Mohammed Abid Service de Recherche, Laboratoire de biologie moléculaire, Institut Pasteur du Maroc, Tanger
Keywords: Epidemiology, Salmonella, PFGE, antimicrobial resistance, phage typing

Abstract

Objectives: The objective of this investigation was to assess the methods for the characterization of Salmonella isolates and to identify relationships of Salmonella isolates from human and food sources in northern Morocco. Methodology: Several Salmonella serotypes were isolated from human and food samples and were characterized using conventional culture methods, biochemical, serological, antimicrobial testing, and phage typing. Molecular analyses such as enterobacterial repetitive intergenic consensus (ERIC)-PCR, macrorestriction profiling by pulsed-field gel electrophoresis (PFGE), and virulence gene analysis were also performed. Results: Sixteen Salmonella strains were isolated in our laboratory, serotyped and identified as S. Kottbus, S. Indiana, S. London, S. Typhi, S. Hadar, S. Corvallis, S. Mbandaka, S. Ouakam, S. Tm var. cop., S. Virchow, and S. Altona. The most common resistance profiles for the isolates was ATCFATSCGKSSS, belonging to phage type PT20, ATASCSS associated with strains DT104L/ad and ATATSS for isolates that were not typeable. The PFGE patterns were different for each Salmonella serotype. All strains were negative for the virulence gene spvR. Conclusions: To our knowledge, this is the first molecular characterization of Salmonella in food and humans from Morocco. Comparison of molecular techniques for differentiating between human and food isolates of Salmonella in north of Morocco shows that ERIC typing and PFGE were more discriminating than the other techniques used in this study.
Published
2009-02-01
How to Cite
1.
Ammari S, Laglaoui A, En-naneiL, Bertrand S, Wildemauwe C, Barrijal S, Abid M (2009) Isolation, drug resistance and molecular characterization of Salmonella isolates in northern Morocco. J Infect Dev Ctries 3:041-049. doi: 10.3855/jidc.104
Section
Original Articles