Serodiagnosis of tuberculous lymphadenitis using a combination of antigens

Authors

  • Demissew Beyene Armauer Hansen Research Institute
  • Kees Lumc Franken 8Department of Infectious Diseases, Leiden University Medical Center, Leiden
  • Lawrence Yamuah Armauer Hansen Research Institute, Addis Ababa
  • Abraham Aseffa Armauer Hansen Research Institute, Addis Abeba
  • Harald G Wiker Section for Microbiology and Immunology, The Gade Institute, University of Bergen Department of Microbiology and Immunology, Haukeland University Hospital, Bergen
  • Arend Kolk The Royal Tropical Institute (KIT), Biomedical Research, Amsterdam
  • Howard Engers 1Armauer Hansen Research Institute, Addis Ababa
  • Paul Klatser The Royal Tropical Institute (KIT), Biomedical Research, Amsterdam
  • Lisbet Sviland Department of Pathology, Haukeland University Hospital, Bergen, Norway and Section for Pathology, The Gade Institute, University of Bergen

DOI:

https://doi.org/10.3855/jidc.116

Keywords:

Tuberculous lymphadenitis, serological test, antigens

Abstract

Background: The diagnosis of extra-pulmonary tuberculosis (EPTB) by conventional methods such as culture and microscopy has low sensitivity and requires an invasive procedure. A simple rapid serological test would be of great value.

Methodology: Six antigens (ESAT-6, Ag85A, TB10.4, Rv3881c, lipoarabinomannan (LAM) and Ara6-BSA) were tested in an ELISA to detect antigen-specific IgG and IgM antibodies in sera from 54 culture- and histology-confirmed tuberculous lymphadenitis (TBLN) patients as follows: four were HIV seropositive; sera from 25 was smear positive for pulmonary tuberculosis (PTB); 15 were culture- and histology-negative lymphadenitis (non-TBLN) patients; and 22 werehealthy controls (HCs).

Results: The sensitivities of the antigens for the detection of IgG in sera of TBLN patients ranged from 4% to 30%. Specificities ranged from 73% to 100% with sera from non-TBLN patients and 91% to 100% with sera from HCs. Sensitivities of the antigens for detection of IgM ranged from 0% to 15% and specificities ranged from 80% to 100% with sera from non-TBLN patients and 91% to 100% with sera from HCs. LAM was the most potent antigen for detection of IgG. When LAM and ESAT-6 were combined, sensitivity increased up to 43% and specificity with non-TBLN was 80% with HC 96%.

Conclusions: The study suggests that the combined use of LAM and ESAT-6 for IgG antibody detection in sera from TBLN patients could be a supplement to microscopy of fine-needle aspirate (FNA) to diagnose TBLN among patients suspected of TBLN.

Author Biographies

Demissew Beyene, Armauer Hansen Research Institute

Deprtment of Molecular Biology and Immunology,

 

Researcher

 

 

Kees Lumc Franken, 8Department of Infectious Diseases, Leiden University Medical Center, Leiden

Lawrence Yamuah, Armauer Hansen Research Institute, Addis Ababa

Abraham Aseffa, Armauer Hansen Research Institute, Addis Abeba

Armauer Hansen Research Institute, Addis Abeba, Ethiopia

Harald G Wiker, Section for Microbiology and Immunology, The Gade Institute, University of Bergen Department of Microbiology and Immunology, Haukeland University Hospital, Bergen

Arend Kolk, The Royal Tropical Institute (KIT), Biomedical Research, Amsterdam

Howard Engers, 1Armauer Hansen Research Institute, Addis Ababa

Paul Klatser, The Royal Tropical Institute (KIT), Biomedical Research, Amsterdam

Lisbet Sviland, Department of Pathology, Haukeland University Hospital, Bergen, Norway and Section for Pathology, The Gade Institute, University of Bergen

Department of Pathology, Haukeland University Hospital, Bergen, Norway and Section for Pathology, The Gade Institute, University of Bergen, Norway

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Published

2010-02-26

How to Cite

1.
Beyene D, Lumc Franken K, Yamuah L, Aseffa A, Wiker HG, Kolk A, Engers H, Klatser P, Sviland L (2010) Serodiagnosis of tuberculous lymphadenitis using a combination of antigens. J Infect Dev Ctries 4:096–102. doi: 10.3855/jidc.116

Issue

Vol. 4 No. 02: February 2010

Section

Original Articles

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