Prevalence of enterotoxigenic and Shiga toxin-producing Escherichia coli in pigs slaughtered in Mato Grosso, Brazil

  • Rodrigo Prado Martins Universidade Federal de Mato Grosso, Brazil
  • Maria Cristina da Silva Universidade Federal de Mato Grosso, Brazil
  • Valeria Dutra Universidade Federal de Mato Grosso, Brazil
  • Luciano Nakazato Universidade Federal de Mato Grosso, Brazil
  • Domingos da Silva Leite Instituto de Biologia, Universidade Estadual de Campinas, Brazil
Keywords: STEC, ETEC, PCR, slaughterhouse, Mato Grosso.

Abstract

Introduction: This study aimed to estimate the prevalence of enterotoxigenic Escherichia coli (ETEC) and Shiga toxin-producing Escherichia coli (STEC) strains in pigs slaughtered in abattoirs located in the state of Mato Grosso, Brazil.

Methodology: Intestinal samples from 74 animals were aseptically dissected and lumen content was plated on MacConkey agar. Confluent colonies from each plate were screened for the presence of ETEC and STEC strains by PCR assays.

Results: It was verified that the prevalence of STEC and ETEC carriers was 1.35% and 9.46% respectively. One (1.35%) of the 74 samples tested was positive for the stx2 gene, and seven (9.46%) for st1, of which two (2.70%) were also positive for lt1.

Conclusion: The results provided represent a benchmark for future research on pathogenic E. coli of porcine origin in Mato Grosso. 


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Published
2010-07-19
How to Cite
1.
Martins RP, da SilvaMC, Dutra V, Nakazato L, LeiteD da S (2010) Prevalence of enterotoxigenic and Shiga toxin-producing Escherichia coli in pigs slaughtered in Mato Grosso, Brazil. J Infect Dev Ctries 5:123-127. doi: 10.3855/jidc.1217
Section
Brief Original Articles