Development of a rapid isothermal amplification method for clinical detection of Vibrio cholerae

Yan Shen; Si-Ming Lu; Hong-Liang Liu; Jia-Long Wang; Yang Li; Li-Guo Liang

doi:10.3855/jidc.21672

Development of a rapid isothermal amplification method for clinical detection of Vibrio cholerae

Authors

Yan Shen Department of Laboratory Medicine, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China
Si-Ming Lu Department of Laboratory Medicine, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China
Hong-Liang Liu Shandong HuiFa Food Co. Ltd, Weifang 262200, Shandong, China
Jia-Long Wang Centre for Clinical Laboratory, The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine), Hangzhou, 310006, China
Yang Li Centre for Clinical Laboratory, The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine), Hangzhou, 310006, China
Li-Guo Liang Centre for Clinical Laboratory, The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine), Hangzhou, 310006, China https://orcid.org/0000-0002-4943-5166

DOI:

https://doi.org/10.3855/jidc.21672

Keywords:

Vibrio cholerae, RPA, POCT, diagnosis, ctxA

Abstract

Introduction: The aim of this study was to develop a rapid isothermal amplification assay for Vibrio cholerae.

Methodology: Specific primers were designed following the guidelines of the National Center for Biotechnology Information (NCBI) database and based on the highly conserved sequence of the Vibrio cholerae virulence factor gene ctxA. A detection method was then developed using recombinase polymerase amplification (RPA). Sensitivity, specificity, clinical sample collection, and processing were calculated.

Results: A rapid isothermal amplification system was developed, showing a detection capability of one copy per microliter. Specificity analysis against a panel of control strains yielded no false-positive results under the tested conditions. The presence of V. cholerae was detected in 9 fecal samples collected from 20 patients who were hospitalized with suspected cholera infection.

Conclusions: This method can be an effective aid in the detection of bacteria in clinical samples.

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Published

2026-08-31

How to Cite

Shen Y, Lu S-M, Liu H-L, Wang J-L, Li Y, Liang L-G (2026) Development of a rapid isothermal amplification method for clinical detection of Vibrio cholerae. J Infect Dev Ctries 20:1235–1240. doi: 10.3855/jidc.21672

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Issue

Vol. 20 No. 08: August 2026

Section

Original Articles

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