Analysis of interleukin-10 gene polymorphisms and hepatitis C susceptibility in Pakistan

Authors

  • Muhammad Sohail Afzal NUST Center of Virology and Immunology, National University of Science and Technology, Sec H-12 Islamabad, Pakistan
  • Sadia Tahir NUST Center of Virology and Immunology, National University of Science and Technology, Sec H-12 Islamabad, Pakistan
  • Amna Salman NUST Center of Virology and Immunology, National University of Science and Technology, Sec H-12 Islamabad, Pakistan
  • Tahir Ahmed Baig NUST Center of Virology and Immunology, National University of Science and Technology, Sec H-12 Islamabad, Pakistan
  • Talha Shafi NUST Center of Virology and Immunology, National University of Science and Technology, Sec H-12 Islamabad, Pakistan
  • Najm Us Sahar Sadaf Zaidi NUST Center of Virology and Immunology, National University of Science and Technology, Sec H-12 Islamabad, Pakistan
  • Ishtiaq Qadri NUST Center of Virology and Immunology, National University of Science and Technology, Sec H-12 Islamabad, Pakistan

DOI:

https://doi.org/10.3855/jidc.1338

Keywords:

IL-10, Polymorphism, HCV

Abstract

Introduction: Hepatitis C virus (HCV) commonly causes a chronic infection but few of patients are able to clear the virus naturally. Interleukin-10 (IL-10) is an anti-inflammatory cytokine that can suppress the immune response against HCV. Interindividual variations in IL-10 production are genetically contributed by polymorphisms within the IL-10 promoter region. This study aimed to investigate the association of the IL-10 gene promoter -1082 G/A, -819 C/T, and -592 C/A polymorphisms with HCV infection susceptibility in Pakistani individuals.

Methodology: Eighty-nine chronically infected patients and 99 controls were enrolled in the study. IL-10 (-1,082 G/A, -819 C/T, -592 C/A) genotyping was performed by amplification refractory mutation system-polymerase chain reaction (ARMS-PCR).

Results: A suggestive evidence of association with hepatitis C was obtained for the IL-10 -819 C/T (-592 C/A) (p: 0.03) promoter polymorphism at the allele level  but not in genotype distribution. The IL-10 -1082 allele showed no association while positive association of GG (p: 0.001) gene and negative association for GA (0.001) gene were observed.  Higher frequencies were observed for GTA (p: 0.02), ACC (p: 0.01) haplotype and GCC/GTA (p: 0.005) diplotype in HCV patients than controls while diplotype GCC/ATA showed protective effect against HCV.

Conclusions: Our findings suggest that different IL-10 gene polymorphisms may lead to an imbalance between the pro-inflammatory and anti-inflammatory cytokine responses which may in turn influence the susceptibility to HCV infection.

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Author Biographies

Muhammad Sohail Afzal, NUST Center of Virology and Immunology, National University of Science and Technology, Sec H-12 Islamabad, Pakistan

Phd Scholar

NUST Center of Virology & Immunology, National University of Science & Technology

Sadia Tahir, NUST Center of Virology and Immunology, National University of Science and Technology, Sec H-12 Islamabad, Pakistan

Phd Scholar

NUST Center of Virology & Immunology, National University of Science & Technology

Amna Salman, NUST Center of Virology and Immunology, National University of Science and Technology, Sec H-12 Islamabad, Pakistan

Phd Scholar

NUST Center of Virology & Immunology, National University of Science & Technology

Tahir Ahmed Baig, NUST Center of Virology and Immunology, National University of Science and Technology, Sec H-12 Islamabad, Pakistan

Senior Research Officer

NUST Center of Virology & Immunology, National University of Science & Technology

Talha Shafi, NUST Center of Virology and Immunology, National University of Science and Technology, Sec H-12 Islamabad, Pakistan

BS student

NUST Center of Virology & Immunology, National University of Science & Technology

Najm Us Sahar Sadaf Zaidi, NUST Center of Virology and Immunology, National University of Science and Technology, Sec H-12 Islamabad, Pakistan

Assistant professor

NUST Center of Virology & Immunology, National University of Science & Technology

Ishtiaq Qadri, NUST Center of Virology and Immunology, National University of Science and Technology, Sec H-12 Islamabad, Pakistan

Professor

NUST Center of Virology & Immunology, National University of Science & Technology

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Published

2011-01-24

How to Cite

1.
Afzal MS, Tahir S, Salman A, Baig TA, Shafi T, Zaidi NUSS, Qadri I (2011) Analysis of interleukin-10 gene polymorphisms and hepatitis C susceptibility in Pakistan. J Infect Dev Ctries 5:473–479. doi: 10.3855/jidc.1338

Issue

Vol. 5 No. 06: June 2011

Section

Original Articles

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