Analysis of interleukin-10 gene polymorphisms and hepatitis C susceptibility in Pakistan

  • Muhammad Sohail Afzal NUST Center of Virology and Immunology, National University of Science and Technology, Sec H-12 Islamabad, Pakistan
  • Sadia Tahir NUST Center of Virology and Immunology, National University of Science and Technology, Sec H-12 Islamabad, Pakistan
  • Amna Salman NUST Center of Virology and Immunology, National University of Science and Technology, Sec H-12 Islamabad, Pakistan
  • Tahir Ahmed Baig NUST Center of Virology and Immunology, National University of Science and Technology, Sec H-12 Islamabad, Pakistan
  • Talha Shafi NUST Center of Virology and Immunology, National University of Science and Technology, Sec H-12 Islamabad, Pakistan
  • Najm Us Sahar Sadaf Zaidi NUST Center of Virology and Immunology, National University of Science and Technology, Sec H-12 Islamabad, Pakistan
  • Ishtiaq Qadri NUST Center of Virology and Immunology, National University of Science and Technology, Sec H-12 Islamabad, Pakistan
Keywords: IL-10, Polymorphism, HCV

Abstract

Introduction: Hepatitis C virus (HCV) commonly causes a chronic infection but few of patients are able to clear the virus naturally. Interleukin-10 (IL-10) is an anti-inflammatory cytokine that can suppress the immune response against HCV. Interindividual variations in IL-10 production are genetically contributed by polymorphisms within the IL-10 promoter region. This study aimed to investigate the association of the IL-10 gene promoter -1082 G/A, -819 C/T, and -592 C/A polymorphisms with HCV infection susceptibility in Pakistani individuals.

Methodology: Eighty-nine chronically infected patients and 99 controls were enrolled in the study. IL-10 (-1,082 G/A, -819 C/T, -592 C/A) genotyping was performed by amplification refractory mutation system-polymerase chain reaction (ARMS-PCR).

Results: A suggestive evidence of association with hepatitis C was obtained for the IL-10 -819 C/T (-592 C/A) (p: 0.03) promoter polymorphism at the allele level  but not in genotype distribution. The IL-10 -1082 allele showed no association while positive association of GG (p: 0.001) gene and negative association for GA (0.001) gene were observed.  Higher frequencies were observed for GTA (p: 0.02), ACC (p: 0.01) haplotype and GCC/GTA (p: 0.005) diplotype in HCV patients than controls while diplotype GCC/ATA showed protective effect against HCV.

Conclusions: Our findings suggest that different IL-10 gene polymorphisms may lead to an imbalance between the pro-inflammatory and anti-inflammatory cytokine responses which may in turn influence the susceptibility to HCV infection.

Author Biographies

Muhammad Sohail Afzal, NUST Center of Virology and Immunology, National University of Science and Technology, Sec H-12 Islamabad, Pakistan

Phd Scholar

NUST Center of Virology & Immunology, National University of Science & Technology

Sadia Tahir, NUST Center of Virology and Immunology, National University of Science and Technology, Sec H-12 Islamabad, Pakistan

Phd Scholar

NUST Center of Virology & Immunology, National University of Science & Technology

Amna Salman, NUST Center of Virology and Immunology, National University of Science and Technology, Sec H-12 Islamabad, Pakistan

Phd Scholar

NUST Center of Virology & Immunology, National University of Science & Technology

Tahir Ahmed Baig, NUST Center of Virology and Immunology, National University of Science and Technology, Sec H-12 Islamabad, Pakistan

Senior Research Officer

NUST Center of Virology & Immunology, National University of Science & Technology

Talha Shafi, NUST Center of Virology and Immunology, National University of Science and Technology, Sec H-12 Islamabad, Pakistan

BS student

NUST Center of Virology & Immunology, National University of Science & Technology

Najm Us Sahar Sadaf Zaidi, NUST Center of Virology and Immunology, National University of Science and Technology, Sec H-12 Islamabad, Pakistan

Assistant professor

NUST Center of Virology & Immunology, National University of Science & Technology

Ishtiaq Qadri, NUST Center of Virology and Immunology, National University of Science and Technology, Sec H-12 Islamabad, Pakistan

Professor

NUST Center of Virology & Immunology, National University of Science & Technology

Published
2011-01-24
How to Cite
1.
Afzal MS, Tahir S, Salman A, Baig TA, Shafi T, Zaidi NUSS, Qadri I (2011) Analysis of interleukin-10 gene polymorphisms and hepatitis C susceptibility in Pakistan. J Infect Dev Ctries 5:473-479. doi: 10.3855/jidc.1338
Section
Original Articles