Evaluation of conventional molecular diagnosis of Mycobacterium tuberculosis in clinical specimens from Morocco

Authors

  • Fathiah Zakham Institut National d’Hygiène, Rabat, Morocco
  • Halima Bazoui Institut National d’Hygiène, Rabat, Morocco
  • Mohammed Akrim Institut National d’Hygiène, Rabat, Morocco
  • Sanae Lemrabet Institut National d’Hygiène, Rabat, Morocco
  • Ouafae Lahlou Institut National d’Hygiène, Rabat, Morocco
  • Mohamed Elmzibri Centre National de l’Energie, des Sciences et Techniques Nucléaires, Rabat, Morocco
  • Abdelaziz Benjouad Laboratoire de Biochimie et Immunologie, Faculté des Sciences. Université Mohammed V-Agdal. Rabat, Morocco
  • My Mustapha Ennaji Centre National de l’Energie, des Sciences et Techniques Nucléaires, Rabat, Morocco
  • Rajae Elaouad Institut National d’Hygiène, Rabat, Morocco

DOI:

https://doi.org/10.3855/jidc.1857

Keywords:

Mycobacterim tuberculosis, polymerase chain reaction, PCR, hsp65 target gene, diagnosis

Abstract

Introduction: Tuberculosis is a major public health threat, annually affecting new individuals worldwide, especially those in developing countries. Rapid detection of the agent and effective treatment are two important factors in controlling this disease.
Methodology: The present study aimed to evaluate polymerase chain reaction (PCR) as a rapid and direct molecular method for the diagnosis of Mycobacterium tuberculosis (MTB) in 70 clinical specimens (62 sputum samples, six cerebrospinal fluids, and two biopsies) using heat shock protein (hsp65) as the gene target. Automated sequencing of the same gene was used for the identification of MTB to the species level.
Results: The sensitivity of PCR was 81.13%, with specificity of 88.24%; the positive and negative predictive values were 95.56% and 60%, respectively.
Conclusion: Based on these results, the hsp65 gene sequence can be used to differentiate the members of MTB complex from non-tuberculosis mycobacteria (NTM).

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Published

2011-11-29

How to Cite

1.
Zakham F, Bazoui H, Akrim M, Lemrabet S, Lahlou O, Elmzibri M, Benjouad A, Ennaji MM, Elaouad R (2011) Evaluation of conventional molecular diagnosis of Mycobacterium tuberculosis in clinical specimens from Morocco. J Infect Dev Ctries 6:40–45. doi: 10.3855/jidc.1857

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Section

Original Articles