Prevalence, diversity and disease association of Helicobacter pylori in dyspeptic patients from Pakistan

  • Adnan Khan Immunology and Infectious Diseases Research Laboratory, Department of Microbiology, University of Karachi, Pakistan
  • Amber Farooqui Division of Immunology, International Institute of Infection and Immunity, Shantou University Medical College, Xinling Road 22, Shantou, Guangdong 515041
  • Yasir Raza Immunology and Infectious Diseases Research Laboratory, Department of Microbiology, University of Karachi
  • Faisal Rasheed Immunology and Infectious Diseases Research Laboratory, Department of Microbiology, University of Karachi
  • Hamid Manzoor Department of Surgery, Civil Hospital, Karachi
  • Syed Shakeel Akhtar Department of Surgery, Civil Hospital, Karachi
  • Muhammad Saeed Quraishy Department of Surgery, Civil Hospital, Karachi
  • Salvatore Rubino Department of Biomedical Sciences, University of Sassari, Sassari, Italy
  • Shahana U Kazmi Immunology and Infectious Diseases Research Laboratory, Department of Microbiology, University of Karachi
  • Bianca Paglietti Department of Biomedical Sciences, University of Sassari, Sassari,
Keywords: H. pylori, cagA, vacA, duodenal ulcer, gastric ulcer, Pakistan

Abstract

Introduction: The etiological association of Helicobacter pylori with gastric ulcer (GU), gastric cancer (GC), and duodenal ulcer (DU) is well-known. Understanding the epidemiology of H. pylori facilitates the estimation of disease burden in a certain population. This study presents the diversity of H. pylori genotypes and their association with different clinical outcomes among dyspeptic patients in Pakistan over a period of four years.

Methodology: Gastric biopsy samples from a total of 450 dyspeptic individualswere subjected to PCR, genotypingand histology.

Results: A total of 201 (45%) cases were found positive for H. pylori. The detection rate was high in GU (91%), DU (86%) and GC (83%) cases compared with those cases who had intact gastric mucosa (18%). Histology revealed the presence of infection in 68% of cases of mild/chronic nonspecific gastritis with others belonging to the GU sequel. cagA gene carriage was observed in 104 (51%) cases or mostly from DU, GU and GC groups, of which 97 were Western type strains while 3 were East-Asian type strains that are rarely observed in South Asia. vacA allelic variant s1am1 was most commonly observed, followed by s1am2, and s1bm1, with direct correlation in diseased cases (gastritis, GU, DU and GC). Prevalent genotypic combinations were s1am1/cagA- in gastritis and s1am1/cagA+ in DU, GU, and GC.

Conclusions: Our study indicates the predominant circulation of Western type cagA and vacAs1am1 type H. pylori strains in Pakistan.

Author Biographies

Adnan Khan, Immunology and Infectious Diseases Research Laboratory, Department of Microbiology, University of Karachi, Pakistan

Lecturer

Department of Microbiology

Muhammad Saeed Quraishy, Department of Surgery, Civil Hospital, Karachi
Published
2013-03-14
How to Cite
1.
Khan A, Farooqui A, Raza Y, Rasheed F, Manzoor H, Akhtar SS, Quraishy MS, Rubino S, Kazmi SU, Paglietti B (2013) Prevalence, diversity and disease association of Helicobacter pylori in dyspeptic patients from Pakistan. J Infect Dev Ctries 7:220-228. doi: 10.3855/jidc.2942
Section
Original Articles

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