Molecular characterization and antibiotic resistance of Salmonella in children with acute gastroenteritis in Abuja, Nigeria

  • Casmir Ifeanyichukwu Cajetan Ifeanyi Faculty of Veterinary Medicine, University of Abuja, Abuja, Nigeria
  • Bassey Enya Bassey World Health Organization Office, Abuja, Nigeria
  • Nkiruka Florence Ikeneche College of Health Science, University of Abuja, Abuja, Nigeria
  • Nazek Al-Gallas Institut Pasteur de Tunis, Tunisie
Keywords: acute gastroenteritis in children, pulse-field gel electrophoresis, Salmonella, antibiotic resistance, Nigeria

Abstract

Introduction: In Nigeria, acute gastroenteritis in children under five years of age is a major cause of mortality and morbidity; identification and characterization of microbial agents of acute gastroenteritis, including Salmonella, remains a powerful tool for effective management, surveillance, and control.

Methodology: Diarrheal stool samples were directly plated onto differential and selective media to isolate Salmonella. Extended-spectrum beta-lactamases were screened using the double disk diffusion technique and by PCR targeting the blaTEM and blaCTX-M genes. Pulsed-field gel electrophoresis (PFGE) was performed usingthe PulseNet Canada Laboratory protocol for molecular subtyping using the restriction enzymes XbaI and BlnI.

Results: The serotypes identified were Salmonella enterica serovar Zanzibar (n = 5), Salmonella Brancaster (n = 3), and one isolate of Salmonella Enteritidis (phage type 1). The following levels of resistance were found among the Salmonella strains: amoxicillin, five strains (55.6%); amoxicillin-clavulanic acid, two strains (22.2%); cephalexin, five strains (55.6%); and cefuroxime, five strains (55.6%). Intermediate resistance was found in five strains (55.6%) only to amoxicillin-clavulanic acid. All isolates were susceptible to nalidixic acid, ciprofloxacin, and ceftriaxone, and no ESBL-producing Salmonella were detected.

Conclusions: Our findings demonstrated the involvement of three Salmonella serovars in acute gastroenteritis; resistance to penicillins and cephalosporins was common.

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Author Biographies

Casmir Ifeanyichukwu Cajetan Ifeanyi, Faculty of Veterinary Medicine, University of Abuja, Abuja, Nigeria
Research Scientist, Department of Veterinary Microbiology, Faculty of Veterinary Medicine, University of Abuja Nigeria
Bassey Enya Bassey, World Health Organization Office, Abuja, Nigeria
Nationl Survilance Officer, World Health Organization Office, Plot 1620, Yusuf Maitama Sule Street, Off Yakubu Gowon Crescent Asokoro, Abuja.
Nkiruka Florence Ikeneche, College of Health Science, University of Abuja, Abuja, Nigeria
Department of Medical Microbiology, College of Health Science, University of Abuja, P.M.B 117, Abuja, Nigeria.
Nazek Al-Gallas, Institut Pasteur de Tunis, Tunisie
Laboratoire de Controˆle des Eaux et Denre´es Alimentaires, Institut Pasteur de Tunis, Tunisie.
Published
2014-06-11
How to Cite
1.
Ifeanyi CIC, Bassey BE, Ikeneche NF, Al-GallasN (2014) Molecular characterization and antibiotic resistance of Salmonella in children with acute gastroenteritis in Abuja, Nigeria. J Infect Dev Ctries 8:712-719. doi: 10.3855/jidc.4185
Section
Original Articles

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