Molecular surveillance of Dengue in Sukabumi, West Java province, Indonesia

  • Roy Nusa Vector Borne Disease Control, Research and Development Council, Ministry of Health of the Republic of Indonesia, Ciamis, West Java, Indonesia
  • Heni Prasetyowati Vector Borne Disease Control, Research and Development Council, Ministry of Health of the Republic of Indonesia, Ciamis, West Java, Indonesia
  • Febrina Meutiawati Eijkman Institute for Molecular Biology, Jakarta, Indonesia
  • Benediktus Yohan Eijkman Institute for Molecular Biology, Jakarta, Indonesia
  • Hidayat Trimarsanto Eijkman Institute for Molecular Biology, Jakarta, Indonesia
  • Tri Yuli Setianingsih Eijkman Institute for Molecular Biology, Jakarta, Indonesia
  • R. Tedjo Sasmono Eijkman Institute for Molecular Biology, Jakarta, Indonesia
Keywords: dengue, surveillance, serotype, genotype

Abstract

Introduction: Dengue is endemic and affects people in all Indonesian provinces. Increasing dengue cases have been observed every year in Sukabumi in West Java province. Despite the endemicity, limited data is available on the genetic of dengue viruses (DENV) circulating in the country. To understand the dynamics of dengue disease, we performed molecular and serological surveillance of dengue in Sukabumi.

Methodology: A total of 113 patients were recruited for this study. Serological data were obtained using anti-dengue IgM and IgG tests plus dengue NS1 antigen detection. Dengue detection and serotyping were performed using real-time RT-PCR. Viruses were isolated and the envelope genes were sequenced. Phylogenetic and evolutionary analyses were performed to determine the genotype of the viruses and their evolutionary rates.

Results: Real-time RT-PCR detected DENV in 25 (22%) of 113 samples. Serotyping revealed the predominance of DENV-2 (16 isolates, 64%), followed by DENV-1 (5 isolates, 20%), and DENV-4 (4 isolates, 16%). No DENV-3 was detected in the samples. Co-circulation of genotype I and IV of DENV-1 was observed. The DENV-2 isolates all belonged to the Cosmopolitan genotype, while DENV-4 isolates were grouped into genotype II. Overall, their evolutionary rates were similar to DENV from other countries.

Conclusions: We revealed the distribution of DENV serotypes and genotypes in Sukabumi. Compared to data obtained from other cities in Indonesia, we observed the differing predominance of DENV serotypes but similar genotype distribution, where the infecting viruses were closely related with Indonesian endemic viruses isolated previously.

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Published
2014-06-11
How to Cite
1.
Nusa R, Prasetyowati H, Meutiawati F, Yohan B, Trimarsanto H, Setianingsih TY, Sasmono RT (2014) Molecular surveillance of Dengue in Sukabumi, West Java province, Indonesia. J Infect Dev Ctries 8:733-741. doi: 10.3855/jidc.3959
Section
Original Articles