Detection and gB genotyping of CMV in Mexican preterm infants in the context of maternal seropositivity

  • José Arellano-Galindo Hospital Infantil de México Federico Gómez Dr. Márquez, México D.F., Mexico
  • Dina Villanueva-García Hospital Infantil de México Federico Gómez Dr. Márquez, México D.F., Mexico
  • José Luis Cruz-Ramirez Hospital de la Mujer, México D.F., Mexico
  • Juan Pablo Yalaupari-Mejìa Hospital de la Mujer, México D.F., Mexico
  • Gabriel Uribe-Gutiérrez Hospital Infantil de México Federico Gómez Dr. Márquez, México D.F., Mexico
  • Norma Velazquez-Guadarrama Hospital Infantil de México Federico Gómez Dr. Márquez, México D.F., Mexico
  • Margarita Nava-Frias Hospital Infantil de México Federico Gómez Dr. Márquez, México D.F., Mexico
  • Onofre Munoz-Hernández Hospital Infantil de México Federico Gómez Dr. Márquez, México D.F., Mexico
  • Juan Manuel Mejía-Arangure Hospital de Pediatría, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico D.F., Mexico
Keywords: seroprevalence, active CMV infection, congenital infection, perinatal infection, gB genotype

Abstract

Introduction: Congenital (CI) and perinatal cytomegalovirus (CMV) infections (PI) can be linked to maternal CMV seropositivity, with fatal consequences in preterm newborns. GB genotyping has been used to analyze genotypic similarity in mothers and infants. The frequency of CMV infection in the context of maternal seropositivity and the viral gB genotypes as well as the genotypic similarity in mothers and preterm infants were investigated.

Methodology: Saliva samples and dry blood spots (DBS) were taken weekly from preterm newborns  from birth  until the first month of life, and breast milk samples were taken from their mothers weekly during the first month of lactation. CMV IgG seroprevalence of the mothers and CI or PI in the infants were established. The gB status and genotypic similarities were established retrospectively in DBS and in the breast milk samples.

Results: In total, 387 neonates and 375 mothers were enrolled. The maternal CMV-positive IgG serology was 97.3% (365/375). Neonatal CMV was found in 5.1% (20/387) of newborns, and one infant presented with CMV-compatible symptoms. CI was 2.5% and PI in the first month after birth was 11.8%. GB2 was the most prevalent genotype and was also the genotype preferentially transmitted to newborns by mothers with mixed infections.

Conclusions: CMV PI and CI in preterm infants from highly seropositive mothers was high, but the rate of symptomatic infection was low. The prevalent genotype was gB2, and this genotype was preferentially transmitted to newborns by mothers with mixed infections.

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Author Biographies

José Arellano-Galindo, Hospital Infantil de México Federico Gómez Dr. Márquez, México D.F., Mexico
Investigador en Ciencias Médicas D
Dina Villanueva-García, Hospital Infantil de México Federico Gómez Dr. Márquez, México D.F., Mexico
Jefe del Servicio de la Unidad de Cuidados Intensivos Neonatales
José Luis Cruz-Ramirez, Hospital de la Mujer, México D.F., Mexico
Jefe de Departamento
Juan Pablo Yalaupari-Mejìa, Hospital de la Mujer, México D.F., Mexico
Jefe del Servicio
Gabriel Uribe-Gutiérrez, Hospital Infantil de México Federico Gómez Dr. Márquez, México D.F., Mexico
Junior research
Norma Velazquez-Guadarrama, Hospital Infantil de México Federico Gómez Dr. Márquez, México D.F., Mexico
Investigador en Ciencias Médicas D
Margarita Nava-Frias, Hospital Infantil de México Federico Gómez Dr. Márquez, México D.F., Mexico
Jefe de Laboratorio
Onofre Munoz-Hernández, Hospital Infantil de México Federico Gómez Dr. Márquez, México D.F., Mexico
Director de Investigación
Juan Manuel Mejía-Arangure, Hospital de Pediatría, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico D.F., Mexico
Jefe de Departamento
Published
2014-06-11
How to Cite
1.
Arellano-GalindoJ, Villanueva-GarcíaD, Cruz-RamirezJL, Yalaupari-MejìaJP, Uribe-GutiérrezG, Velazquez-GuadarramaN, Nava-FriasM, Munoz-HernándezO, Mejía-ArangureJM (2014) Detection and gB genotyping of CMV in Mexican preterm infants in the context of maternal seropositivity. J Infect Dev Ctries 8:758-767. doi: 10.3855/jidc.3501
Section
Original Articles