Gut microbiota related to Giardia duodenalis, Entamoeba spp. and Blastocystis hominis infections in humans from Côte d’Ivoire

  • Valerio Iebba Sapienza University of Rome, Rome, Italy
  • Floriana Santangelo Sapienza University of Rome, Rome, Italy
  • Valentina Totino Sapienza University of Rome, Rome, Italy
  • Fabrizio Pantanella Sapienza University of Rome, Rome, Italy
  • Anatole Monsia Centre Don Orione pour handicapés physiques, Bonoua, Côte d’Ivoire
  • Veronica Di Cristanziano University of Cologne, Cologne, Germany
  • David Di Cave University of Rome Tor Vergata, Rome, Italy
  • Serena Schippa Sapienza University of Rome, Rome, Italy
  • Federica Berrilli University of Rome Tor Vergata, Rome, Italy
  • Rossella D'Alfonso Centre Don Orione pour handicapés physiques, Bonoua, Côte d’Ivoire
Keywords: microbiota, Giardia duodenalis, Entamoeba spp., Blastocystis hominis, dysbiosis, Côte d’Ivoire

Abstract

Introduction: Literature data provide little information about protozoa infections and gut microbiota compositional shifts in humans. This preliminary study aimed to describe the fecal bacterial community composition of people from Côte d’Ivoire harboring Giardia duodenalis, Entamoeba spp., and Blastocystis hominis, in trying to discover possible alterations in their fecal microbiota structure related to the presence of such parasites.

Methodology: Twenty fecal samples were collected from people inhabiting three different localities of Côte d’Ivoire for copromicroscopic analysis and molecular identification of G. duodenalis, Entamoeba spp., and B. hominis. Temporal temperature gradient gel electrophoresis (TTGE) was used to obtain a fingerprint of the overall bacterial community; quantitative polymerase chain reaction (qPCR) was used to define the relative abundances of selected bacterial species/group, and multivariate statistical analyses were employed to correlate all data.

Results: Cluster analysis revealed a significant separation of TTGE profiles into four clusters (p < 0.0001), with a marked difference for G. duodenalis-positive samples in relation to the others (p = 5.4×10-6). Interestingly, qPCR data showed how G. duodenalis-positive samples were related to a dysbiotic condition that favors potentially harmful species (such as Escherichia coli), while Entamoeba spp./B. hominis-positive subjects were linked to a eubiotic condition, as shown by a significantly higher Faecalibacterium prausnitzii-Escherichia coli ratio.

Conclusions: This preliminary investigation demonstrates a differential fecal microbiota structure in subjects infected with G. duodenalis or Entamoeba spp./B. hominis, paving the way for using further next-generation DNA technologies to better understand host-parasite-bacteria interactions, aimed at identifying potential indicators of microbiota changes.

Published
2016-09-30
How to Cite
1.
Iebba V, Santangelo F, Totino V, Pantanella F, Monsia A, Di Cristanziano V, Di Cave D, Schippa S, Berrilli F, D’Alfonso R (2016) Gut microbiota related to Giardia duodenalis, Entamoeba spp. and Blastocystis hominis infections in humans from Côte d’Ivoire. J Infect Dev Ctries 10:1035-1041. doi: 10.3855/jidc.8179
Section
Brief Original Articles