Molecular typing and drug resistance analysis of carbapenem-resistant Klebsiella pneumoniae from paediatric patients in China

Authors

  • Xian Zhang Department of Hospital Infection, The Third Affiliated Hospital of Zunyi Medical University, The First People's Hospital of Zunyi, Guizhou, China
  • Jian Xue Department of Health Management, Zunyi Medical And Pharmaceutical College, Guizhou, China
  • Mei-Jing Shen Department of Clinical Laboratory, The Third Affiliated Hospital of Zunyi Medical University, The First People's Hospital of Zunyi, Guizhou, China
  • Wen-Hong An Department of Hospital Infection, The Third Affiliated Hospital of Zunyi Medical University, The First People's Hospital of Zunyi, Guizhou, China
  • Ze-Qiong Chen Department of Hospital Infection, The Third Affiliated Hospital of Zunyi Medical University, The First People's Hospital of Zunyi, Guizhou, China
  • Kai-Feng Wu Department of Clinical Laboratory, The Third Affiliated Hospital of Zunyi Medical University, The First People's Hospital of Zunyi, Guizhou, China

DOI:

https://doi.org/10.3855/jidc.17003

Keywords:

Whole genome sequencing, paediatrics, carbapenem-resistant Klebsiella pneumoniae, molecular typing, drug resistance

Abstract

Introduction: There are few studies on paediatric carbapenem-resistant Klebsiella pneumoniae (CRKP) in China. The present study investigated the molecular epidemiological and drug resistance characteristics of CRKP from paediatric patients in China to provide a reference for the prevention and control of CRKP infection.

Methodology: In total, 20 nonrepetitive clinical CRKP isolates were collected between February 2019 and February 2020 in a tertiary hospital in China. Strain identification and drug susceptibility testing were carried out using the VITEK® 2 Compact Bacterial Identification and Monitoring System. Sequence typing, phylogenetic relationships, and antibiotic resistance-associated genes were analysed by whole genome sequencing (WGS).

Results: sequence typing (MLST) and Core genome multilocus sequence typing (cgMLST) analysis revealed the most frequently represented were ST2407-CT3536 (30%), ST76-CT5893 (25%), and ST309-CT7864 (25%). All 20 CRKP isolates were divided into three clusters. All isolates were highly resistant to a variety of β-lactams and were highly susceptible to quinolones, aminoglycosides, and sulphonamides. All isolates mainly carried the carbapenem resistance genes blaNDM-1 and blaKPC-2, among which 10 isolates carried both blaNDM-1 and blaKPC-2 simultaneously.

Conclusions: Sequence typing, phylogenetic relationships, and antibiotic resistance genes can be determined using WGS technology. This can guide CRKP infection control and clinical treatment for paediatric patients.

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Published

2022-11-29

How to Cite

1.
Zhang X, Xue J, Shen M-J, An W-H, Chen Z-Q, Wu K-F (2022) Molecular typing and drug resistance analysis of carbapenem-resistant Klebsiella pneumoniae from paediatric patients in China. J Infect Dev Ctries 16:1726–1731. doi: 10.3855/jidc.17003

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Section

Original Articles