Clinical utility of universal 16S rRNA PCR using sterile body fluids: lung abscess caused by Nocardia farcinica

Authors

  • Jungseo Park College of Medicine, Chosun University, Gwangju, South Korea
  • Jun-Won Seo Graduate School of Chosun University, Gwangju, Republic of Korea
  • Na Ra Yoon Departments of Internal Medicine, College of Medicine, Chosun University, Gwangju, Republic of Korea
  • Dong-Min Kim Departments of Internal Medicine, College of Medicine, Chosun University, Gwangju, Republic of Korea
  • Choon-Mee Kim Premedical Science, College of Medicine, Chosun University, Gwangju, Republic of Korea

DOI:

https://doi.org/10.3855/jidc.15449

Keywords:

16S rRNA, Gram-positive bacteria, polymerase chain reaction, Gram-staining, nocardiosis

Abstract

Nocardiosis is an infectious disease caused by Gram-positive rod-shaped bacteria and presents as a suppurative granulomatous disease in patients with compromised immune systems. Few studies have investigated the clinical utility of the universal 16S rRNA polymerase chain reaction (PCR) method using sterile body fluids for diagnosing nocardiosis.

A 64-year-old female patient was admitted to Chosun University Hospital with the complaint of fever. Computed tomography scans of her chest revealed the presence of empyema and an abscess in the right lung. Pus samples were collected using closed chest thoracostomy and were cultured. The results revealed the presence of Gram-positive bacilli, but the culture tests were unable to identify the causative microorganism. Despite antibiotic treatment, the patient died of the suspected empyema and abscess. Universal 16S PCR of her sterile body fluids in combination with sequencing was performed, which led to the diagnosis of Nocardia farcinica infection. Postmortem, the remainder of the pus samples cultured for 8 days confirmed the presence of N. farcinica. This study illustrates the importance of using routine universal 16S rRNA PCR with sterile body fluids to help diagnose atypical bacterial infections such as nocardiosis.

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Published

2023-05-31

How to Cite

1.
Park J, Seo J-W, Yoon NR, Kim D-M, Kim C-M (2023) Clinical utility of universal 16S rRNA PCR using sterile body fluids: lung abscess caused by Nocardia farcinica. J Infect Dev Ctries 17:716–718. doi: 10.3855/jidc.15449

Issue

Section

Case Reports