Investigation on colorectal cancer and human herpesvirus infection among Algerian patients

Amina Bouchemal; Joan Martí Carreras; Lydia Khireddine; Ahmed Amir Bouchemal; Fares Nasri; Piet Maes; Abderrezak Ghidouche; Fatiha Bedjou

doi:10.3855/jidc.17640

Investigation on colorectal cancer and human herpesvirus infection among Algerian patients

Authors

Amina Bouchemal Université de Bejaia, Faculté des Sciences de la Nature et de la Vie, Laboratoire de Biotechnologies Végétales et Ethnobotanique, Bejaia, Algerie
Joan Martí Carreras Zoonotic Infectious Diseases Unit, Laboratory of Clinical and Epidemiological Virology, Department of Microbiology, Immunology and Transplantation, Rega Institute, KU Leuven, Leuven, Belgium https://orcid.org/0000-0002-0005-9761
Lydia Khireddine Université de Bejaia, Faculté des Sciences de la Nature et de la Vie, Laboratoire de Biotechnologies Végétales et Ethnobotanique, Bejaia, Algerie https://orcid.org/0000-0002-1319-4604
Ahmed Amir Bouchemal Service de chirurgie générale B, Centre Hospitalo Universitaire Constantine, Constantine, Algeria
Fares Nasri Laboratoire d’anatomie pathologique, Centre Hospitalo Universitaire Constantine, Constantine, Algeria
Piet Maes Zoonotic Infectious Diseases Unit, Laboratory of Clinical and Epidemiological Virology, Department of Microbiology, Immunology and Transplantation, Rega Institute, KU Leuven, Leuven, Belgium
Abderrezak Ghidouche Université de Bejaia,Faculté de Medecine,Laboratoire de Génie Biologique des Cancers,Bejaia,Algerie
Fatiha Bedjou Université de Bejaia, Faculté des Sciences de la Nature et de la Vie, Laboratoire de Biotechnologies Végétales et Ethnobotanique, Bejaia, Algerie

DOI:

https://doi.org/10.3855/jidc.17640

Keywords:

herpesviruses, PCR, tumorigenesis, prevalence, CRC

Abstract

Introduction: Herpesviruses are a widespread family of double-stranded DNA viruses that establish life-long persistent infection in their hosts. Cumulative evidence tends to argue for the association of human herpesviruses, such as Kaposi's sarcoma herpesvirus (KHSV), Epstein-Barr virus (EBV), and human cytomegalovirus (HCMV) with various human disorders and diseases. The present study aims to investigate the presence of herpesviruses in colorectal cancer (CRC).

Methodology: We investigated the presence of herpesviruses in 69 formalin-fixed paraffin embedded tissue (FFPE) biopsies, using a pan-herpesvirus nested polymerase chain reaction (PCR) with degenerate primers and HCMV specific primers to identify the presence of herpesviruses in CRC tissue.

Results: None of the samples we examined were positive for herpesviruses.

Conclusions: Our results suggest that there is no (or very low) prevalence of lifelong herpesvirus infection in Algerian CRC patients. Larger cohorts may provide more insight into the prevalence of herpesviruses in Algerian CRC biopsies.

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Published

2023-05-31

How to Cite

Bouchemal A, Martí Carreras J, Khireddine L, Bouchemal AA, Nasri F, Maes P, Ghidouche A, Bedjou F (2023) Investigation on colorectal cancer and human herpesvirus infection among Algerian patients. J Infect Dev Ctries 17:656–664. doi: 10.3855/jidc.17640

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Issue

Vol. 17 No. 05: May 2023

Section

Original Articles

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