Efficacy of boric acid used to treat experimental vascular graft infection by methicillin-resistant Staphylococcus aureus

Özlem Kirişci; Mehmet Kirişci; Tuba Özcan Metin; Duygun Altıntaş Aykan; Murat Aral; Adem Doğaner; Gülsen Bayrak

doi:10.3855/jidc.17865

Efficacy of boric acid used to treat experimental vascular graft infection by methicillin-resistant Staphylococcus aureus

Authors

Özlem Kirişci Department of Microbiology, Faculty of Medicine, Kahramanmaraş Sütçü İmam University, Kahramanmaraş, Turkey https://orcid.org/0000-0003-4784-8183
Mehmet Kirişci Department of Cardiovascular Surgery, Faculty of Medicine, Kahramanmaraş Sütçü İmam University, Kahramanmaraş, Turkey
Tuba Özcan Metin Department of Histology and Embryology, Faculty of Medicine, Kahramanmaraş Sütçü İmam University, Kahramanmaraş, Turkey
Duygun Altıntaş Aykan Department of Pharmacology, Faculty of Medicine, Kahramanmaraş Sütçü İmam University, Kahramanmaraş, Turkey https://orcid.org/0000-0001-8224-4006
Murat Aral Etlik City Hospital, Medical Microbiology Clinic, Ankara, Turkey
Adem Doğaner Department of Biostatistics, Faculty of Medicine, Kahramanmaraş Sütçü İmam University, Kahramanmaraş, Turkey
Gülsen Bayrak Department of Histology and Embryology, Faculty of Medicine, Uşak University, Uşak, Turkey

DOI:

https://doi.org/10.3855/jidc.17865

Keywords:

Teicoplanin, boric acid, S. aureus, cytokines, rat model

Abstract

Introduction: We aimed to investigate the efficacy of local boric acid (BA) and teicoplanin in prosthetic vascular graft infection (PVGI) caused by methicillin-resistant Staphylococcus aureus (MRSA) in a rat model.

Methodology: Fourty rats were divided into five groups. Group 1 received no treatments (control group); group 2 was uncontaminated polytetrafluoroethylene (PTFE) graft group; group 3 was untreated and the PTFE graft was contaminated with 2×10⁷ CFU/mL MRSA; group 4 received local BA (8 mg/kg) and was contaminated with with 2×10⁷ CFU/mL MRSA; group 5 received local BA (8 mg/kg) and intraperitoneal teikoplanin (10 mg/kg), and was contaminated with 2×10⁷ CFU/mL MRSA; On the 3rd day, grafts and serums were removed for microbiological, histological and serological tests.

Results: The amounts of culture growth in groups 4 and 5 were significantly lower compared to group 3 (p < 0.001). TNF-α was significantly higher in Group 3 than the other groups (p = 0.001). There was no significant difference between the groups in serum IL-1 levels (p = 0.138). Monocyte chemotactic protein-1 (MCP-1) was not significantly different between groups 3, 4, and 5, but it was significantly higher than groups 1 and 2 (p < 0.001). The severity of inflammation was significantly higher in group 3 than the other groups, and fibroblastic proliferation, granulation tissue and collagen synthesis were significantly lower (p < 0.05).

Conclusions: Our study showed that local BA and combined teicoplanin treatment is effective in preventing PVGI.

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Published

2023-09-30

How to Cite

Kirişci Özlem, Kirişci M, Özcan Metin T, Altıntaş Aykan D, Aral M, Doğaner A, Bayrak G (2023) Efficacy of boric acid used to treat experimental vascular graft infection by methicillin-resistant Staphylococcus aureus. J Infect Dev Ctries 17:1317–1324. doi: 10.3855/jidc.17865

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Issue

Vol. 17 No. 09: September 2023

Section

Original Articles

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