Molecular typing characteristics and drug resistance of Salmonella from food-borne diarrhea patients in Chifeng, China

Chunru Wei; Yanbo Bai; Huying Li; Peng Peng; Yunyao Zhao; Huixia Yu; Fengjun Yang

doi:10.3855/jidc.21764

Molecular typing characteristics and drug resistance of Salmonella from food-borne diarrhea patients in Chifeng, China

Authors

Chunru Wei Microbiology Laboratory, Key Laboratory of Environmental Medicine, Chifeng Center for Disease Control and Prevention, Chifeng, Inner Mongolia Province, China https://orcid.org/0000-0003-1770-5974
Yanbo Bai Microbiology Laboratory, Key Laboratory of Environmental Medicine, Chifeng Center for Disease Control and Prevention, Chifeng, Inner Mongolia Province, China https://orcid.org/0009-0009-9778-9514
Huying Li Department of Nursing, Zibo Vocational Institute, Zibo, Shandong Province, China https://orcid.org/0000-0001-9976-6839
Peng Peng Microbiology Laboratory, Key Laboratory of Environmental Medicine, Chifeng Center for Disease Control and Prevention, Chifeng, Inner Mongolia Province, China https://orcid.org/0009-0008-6801-4720
Yunyao Zhao Microbiology Laboratory, Key Laboratory of Environmental Medicine, Chifeng Center for Disease Control and Prevention, Chifeng, Inner Mongolia Province, China https://orcid.org/0009-0009-3693-2460
Huixia Yu Microbiology Laboratory, Key Laboratory of Environmental Medicine, Chifeng Center for Disease Control and Prevention, Chifeng, Inner Mongolia Province, China https://orcid.org/0009-0003-0067-9009
Fengjun Yang Microbiology Laboratory, Key Laboratory of Environmental Medicine, Chifeng Center for Disease Control and Prevention, Chifeng, Inner Mongolia Province, China https://orcid.org/0009-0001-5349-1080

DOI:

https://doi.org/10.3855/jidc.21764

Keywords:

foodborne infections, Salmonella, serotype distribution, antimicrobial resistance, PFGE typing

Abstract

Introduction: This study aimed to investigate the infection status, serotype distribution, drug resistance, and molecular typing characteristics of Salmonella in food-borne diarrhea patients in Chifeng, China, from 2022 to 2024.

Methodology: Fecal samples were collected from diarrhea patients for Salmonella isolation and molecular serotype identification. The isolates were tested using the microbroth dilution method and pulsed-field gel electrophoresis (PFGE) to assess drug susceptibility and perform molecular typing. Statistical analysis was conducted using the SPSS 25.0 software.

Results: Salmonella was detected in 52 of the 737 fecal samples, yielding a positive detection rate of 7.06% (52/737). A total of 52 Salmonella strains were isolated, predominantly Salmonella Typhimurium and Salmonella Enteritidis, which accounted for 40.38% (21/52) and 21.15% (11/52), respectively. Additionally, 5 other serotypes were identified: S. Kentucky, S. Dublin, S. Liverpool, S. Vilshaw, and S. Paratyphoid A. Notably, 48 strains (92.31%, 48/52) exhibited resistance to at least one antibiotic, with resistance rates exceeding 50% for ampicillin, streptomycin, tetracycline, and nalidixic acid. The rate of multiple drug resistance reached 86.54% (45/52). Cluster analysis of the 52 Salmonella strains revealed 39 band types with similarity indices ranging from 50.1% to 100%. Notably, a higher similarity coefficient indicated greater similarity in drug resistance phenotypes among the strains.

Conclusions: The detection rate of Salmonella among food-borne diarrhea patients in Chifeng from 2022 to 2024 was notably high. The predominant serotypes were S. Typhimurium and S. Enteritidis. The PFGE band types were relatively diverse, and the strains exhibited significant drug resistance, including multiple drug resistance.

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Published

2026-05-31

How to Cite

Wei C, Bai Y, Li H, Peng P, Zhao Y, Yu H, Yang F (2026) Molecular typing characteristics and drug resistance of Salmonella from food-borne diarrhea patients in Chifeng, China. J Infect Dev Ctries 20:693–702. doi: 10.3855/jidc.21764

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Issue

Vol. 20 No. 05: May 2026

Section

Original Articles

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